能够降解聚乙烯（PE）和聚丙烯（PP）的微生物的筛选,Microbiological R
能够降解聚乙烯（PE）和聚丙烯（PP）的微生物的筛选
Microbiological Research
(
IF
6.1
)
Pub Date : 2022-11-08
, DOI:
10.1016/j.micres.2022.127251
Mariusz Wróbel
1
,
Sonia Szymańska
2
,
Tomasz Kowalkowski
3
,
Katarzyna Hrynkiewicz
2
Affiliation
Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland; Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.
Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland.
微生物降解微塑料，地面清洗13825404095但它们的潜力仍未得到充分利用，例如，由于微生物的选择不充分。我们开发了一种能够降解聚乙烯 (PE) 和聚丙烯 (PP) 的微生物的有效选择方法，并评估了微生物种群降解聚合物的规模。 我们还分离出具有 PE 降解潜力的七种细菌菌株（三种巨大普氏菌菌株、肺炎克雷伯菌、荧光假单胞菌、路德肠杆菌、金黄杆菌属）和七种真菌菌株（四种镰刀菌属、两种Lecanicillium属和木霉属）七种细菌菌株（五种粘质沙雷氏菌和两种肠杆菌属）和六种真菌菌株（四种曲霉属、尖孢镰刀菌和颗粒青霉) 具有PP降解能力。扫描电子显微镜 (SEM) 分析证实了生物膜的存在，并揭示了 PE 和 PP 颗粒的表面变化，但观察到最大变化（微裂纹和波纹）的是与细菌一起孵育的 PP。傅里叶变换红外 (FTIR) 光谱证实了所研究聚合物表面的结构变化。 总之，从垃圾填埋场分离塑料降解细菌和真菌是收集具有高 PE 和 PP 降解潜力的微生物的有效策略。细菌和真菌分别显示出更好的 PP 降解和 PE 降解潜力。
"点击查看英文标题和摘要"
Selection of microorganisms capable of polyethylene (PE) and polypropylene (PP) degradation
Microorganisms degrade microplastics, but their potential is still not fully exploited, e.g., due to inadequate selection of microorganisms. We developed an effective selection method of microorganisms capable of polyethylene (PE) and polypropylene (PP) degradation and assessed the scale of polymer degradation by microbial populations. We isolated seven bacterial strains (three Priestia megaterium strains, Klebsiella pneumoniae, Pseudomonas fluorescens, Enterobacter ludwigii, Chryseobacterium sp.) and seven fungal strains (four Fusarium spp., two Lecanicillium spp. and Trichoderma sp.) with PE degradation potential, as well as seven bacterial strains (five Serratia marcescens and two Enterobacter spp.) and six fungal strains (four Aspergillus spp., Fusarium oxysporum and Penicillium granulatum) with PP degradation ability. Scanning electron microscopy (SEM) analysis confirmed the presence of a biofilm and revealed surface changes in both PE and PP pellets, but the greatest changes (microcracks and corrugations) were observed for PP incubated with bacteria. Fourier transform infrared (FTIR) spectroscopy confirmed the structural changes on the studied polymer surfaces. In conclusion, the isolation of plastic-degrading bacteria and fungi from waste landfills represents an effective strategy for the collection of microorganisms with high potential for PE and PP degradation. The bacteria and fungi revealed better potential for PP degradation and PE degradation, respectively.