保洁清洁全屋打扫清理安保顺家政

Effects of fermented seaweed Sargassum fusiforme on microflora structure in intestinal and fecal differential metabolites in mice

ZHAO Fei, TONG Changqing, LI Wei*, QU Min

(College of Food Science and Engineering, Collaborative Innovation Center of Seafood Deep Processing, Dalian Ocean University, Dalian 116023, China)

Abstract： To explore the diversity structure of intestinal microflora and the changes in differential metabolites in feces of mice administered with fermented seaweed Sargassum fusiforme fluid(FSFF), the intestinal flora, metabolites and metabolic pathways were investigated in the mice administered with the FSFF. The FSFF was prepared by the fact that mixture of the seaweed, apples, pears, carrots and winter squash cut into 1-3 cm squares was fermented at a mass ratio of 15.0 g∶1.0 g∶0.5 g∶0.5 g∶0.5 g by addition 0.5% of each yeast, lactic acid bacteria and acetic acid bacteria in a fermenter at 10-25 ℃ for 50 d, and filtered to make the fluid. Mice were divided into 2 groups: control groups with gastric perfusion of physiological saline and test groups administrated with gastric perfusion of FSFF for 45 days, and then the diversity structure of intestinal microflora and metabolites were investigated in feces of the mice administered with FSFF by 16S rRNA high-throughput sequencing technology, and UPLC-TOF-MS to evaluate the relationship between intestinal flora and metabolites in mice. It was found that the abundance of beneficial bacteria including Lactobacillus and Bifidobacterium in the feces of mice fed with FSFF was increased, and that Bacteroides, Helicobacter and other harmful bacteria decreased in abundance based on the Illumina MiSeq platform, compared with mice in the saline group. The UPLC-TOF-MS fecal metabolomics revealed that 14 fecal metabolites related to the FSFF in the positive ion modes and 14 in negative ion modes were differential contrast to the saline group, and the 7 related metabolic pathways were: purine metabolism, alanine, aspartic acid and glutamic acid metabolism, pyrimidine metabolism, arginine biosynthesis, galactose metabolism, arginine and proline metabolism pathways, and glycine, serine and threonine metabolism, in which purine metabolism, alanine, aspartic acid and glutamic acid metabolism, pyrimidine metabolism, galactose metabolism, arginine and proline metabolism pathways had the most significant effects. There was significant difference in the ratio of beneficial bacteria to pathogenic bacteria in the intestines of mice between the groups, with the hgher proportion of beneficial bacteria in the test groups than that in the control group, and significantly different metabolites. The findings indicated that S.fusiforme enzymes regulated the intestinal flora in the mice.

Key words： Sargassum fusiforme enzyme; mouse; intestinal flora; metabolomics