Surfactin 通过积累细胞内 ROS 并诱导细胞凋亡机制抑制禾谷镰刀菌,World
Surfactin 通过积累细胞内 ROS 并诱导细胞凋亡机制抑制禾谷镰刀菌
World Journal of Microbiology and Biotechnology
(
IF
4.2
)
Pub Date : 2023-10-12
, DOI:
10.1007/s11274-023-03790-2
Chen Liang
1
,
Xu Xi-Xi
1
,
Sun Yun-Xiang
1
,
Xin Qiu-Hua
1
,
Lv Yang-Yong
1
,
Hu Yuan-Sen
1
,
Bian Ke
1
Affiliation
School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
禾谷镰刀菌(Fusarium graminearum)是一种毁灭性真菌病原菌,酒店保洁13825404095是全球小麦赤霉病 (FHB)的主要病原菌;它导致严重的产量损失和霉菌毒素污染,严重威胁全球小麦生产和粮食安全。然而,尽管不断努力,控制这种病原体仍然是一个重大挑战。表面活性剂,主要由芽孢杆菌合成。通过非核糖体肽合成酶,具有有效的表面活性剂和抗菌特性,但其抗真菌机制尚未完全阐明。我们发现表面活性剂对禾谷镰刀菌菌丝生长的EC 50为102.1 µg/mL,田间条件下对小麦赤霉病的防效对小麦品种淮麦40和周麦36分别达到86.38%和81.60%,表明表面活性剂对禾谷镰刀菌菌丝生长的EC 50 为102.1 µg/mL。对禾谷镰刀菌具有潜在的抗真菌活性。禾谷镰刀菌菌丝中表面活性素诱导细胞内ROS积累、线粒体膜电位(MMP)降低、元半胱天冬酶活性激活和染色质浓缩,表明真菌生长抑制主要是由细胞凋亡样细胞死亡引起。此外,积累的细胞内ROS被证明是表面活性素诱导细胞凋亡的关键介质。广谱 caspase 抑制剂 Z-VAD-FMK 处理表明 surfactin 诱导禾谷镰刀菌不依赖 caspase 的细胞凋亡。总的来说,这项研究提供了证据,表明表面活性素可诱导禾谷镰刀菌菌丝中ROS介导的线粒体凋亡,并可能通过激活细胞凋亡来发挥其抗真菌活性。该研究展示了Surfactin作为FHB生物防治抗真菌剂的潜力,为Surfactin抗丝状真菌的抗真菌机制提供了新的视角,并有助于产Surfactin微生物在植物病害生物防治中的应用。
"点击查看英文标题和摘要"
Surfactin inhibits Fusarium graminearum by accumulating intracellular ROS and inducing apoptosis mechanisms
Fusarium graminearum, a devastating fungal pathogen, is the main pathogen of Fusarium head blight (FHB) in wheat globally; it results in significant yield loss and mycotoxin contamination that severely threatens global wheat production and food safety. However, despite ongoing efforts, controlling this pathogen still remains a major challenge. Surfactin, primarily synthesized by Bacillus sp. via non-ribosomal peptide synthetases, exhibits potent surfactant and antibacterial properties, but its antifungal mechanism has yet to be fully elucidated. We found that the EC50 of surfactin against hyphal growth of F. graminearum was 102.1 µg/mL, and control efficacy against wheat FHB under field conditions achieved 86.38% in wheat cultivar Huaimai 40 and 81.60% in wheat cultivar Zhoumai 36, indicating that surfactin has potential antifungal activity against F. graminearum. Accumulated intracellular ROS, decreased mitochondrial membrane potential (MMP), activated metacaspase activity and condensed chromatin, were induced by surfactin in F. graminearum hyphae, suggesting that growth inhibition of fungus is mainly caused by apoptosis-like cell death. Furthermore, accumulated intracellular ROS was evidenced to act as a key mediator of surfactin-induced apoptosis. Broad-spectrum caspase inhibitor Z-VAD-FMK treatment indicated that surfactin induces caspase-independent apoptosis in F. graminearum. Collectively, this study provides evidence that surfactin induces a ROS-mediated mitochondrial apoptosis in F. graminearum hyphae, and may exert its antifungal activity against F. graminearum by activating apoptosis. This study demonstrates the potential of surfactin as an antifungal agent for FHB biocontrol, provides a new perspective on the antifungal mechanism of surfactin against filamentous fungi, and contributes to the application of surfactin-producing microbes in the biocontrol of plant diseases.