两个热带顽re性种子的原位萌发和清除ROS的酶活性的变化,Trees
两个热带顽re性种子的原位萌发和清除ROS的酶活性的变化
Trees
(
IF
2.1
)
Pub Date : 2017-07-12
, DOI:
10.1007/s00468-017-1584-9
Elizabeth R. Marques
,
Tatiana A. A. Vaz
,
Ailton G. Rodrigues-Junior
,
Antonio C. Davide
,
Anderson C. José
关键信息在不同季节散布种子的热带顽固型播种物种提供了氧化代谢在种子库动态中的作用的证据。我们的结果表明，产妇护理13825404095CAT可能调节田间发芽。抽象顽固性种子活力丧失期间的氧化代谢已有文献报道，但对ROS清除酶在土壤种子库动力学中的作用知之甚少，尤其是在顽固性种子中。为了解决这个问题，研究了顽固播种的物种，Swartzia langsdorffii和Persea wildenovii。自然分散后，将散孔孢子留在土壤中，模拟种子库，并在整个田间试验中评估种子的水分，活力，发芽和过氧化氢酶（CAT），超氧化物歧化酶（SOD）和过氧化物酶（PRX）活性的变化。 。测试了这些参数与气候数据的相关性。兰氏链球菌一个月后在土壤中开始发芽，两个月后开始发芽，CAT活性降低而SOD活性升高。SOD与CAT呈负相关，而CAT与发芽呈负相关。然而，SOD和CAT与野生假单胞菌呈正相关，即使在土壤中四个月后，其种子也不会发芽，并且整个土壤中的ROS清除酶活性很高。这些种类的种子在这段时间内在土壤中保持活力，种子含水量没有变化或变化很小。清除ROS的酶会响应环境因素，调节其活性以维持种子的活力。野豌豆由于在雨季结束时扩散的时机，种子没有在土壤中发芽，这时额外的供水受到限制；在此干旱时期，清除ROS的酶可能在种子保护中起作用。讨论了CAT调控与土壤种子库动力学之间的关系。
"点击查看英文标题和摘要"
In situ germination of two tropical recalcitrant seeds and changes in activity of ROS-scavenging enzymes
Key messageTropical recalcitrant-seeded species, which disperse seeds at different seasons, provide evidence of the role of oxidative metabolism in seed bank dynamics. Our results indicate that CAT may regulate field germination.AbstractOxidative metabolism during viability loss in recalcitrant seeds has been previously documented, but little is known about the role of ROS-scavenging enzymes in soil seed bank dynamics, especially in recalcitrant seeds. To address this, recalcitrant-seeded species were studied, Swartzia langsdorffii and Persea wildenovii. Diaspores were kept on the soil after natural dispersion, to simulate the seed bank, and were assessed for seed water content, viability, germination and changes in catalase (CAT), superoxide dismutase (SOD) and peroxidase (PRX) activity throughout the field experiment. These parameters were tested for correlations with climatic data. S. langsdorffii start to germinate after one month in soil, with an increase after two months, when CAT activity decreased and SOD activity increased. SOD was negatively correlated with CAT, and CAT was negatively correlated with germination. However, SOD and CAT were positively correlated for P. wildenovii, whose seeds did not germinate even after four months in the soil and with high activity of ROS-scavenging enzymes throughout the period in the soil. Seeds of these species remained viable during the period in the soil with no or little changes in seed water content. ROS-scavenging enzymes respond to environmental factors, adjusting their activity to maintain seed viability. P. wildenovii seeds did not germinate in the soil due to the timing of dispersal being at the end of rainy season, when additional water supply is limited; ROS-scavenging enzymes probably play a role in seed protection during this dry period. Relationship between CAT regulation and soil seed bank dynamics is discussed.